31.氫水對放射性心臟損傷的治療作用之研究

氫氣的選擇性抗氧化的發現，引起了氫氣生物學研究的熱潮，氫氣治療各類器官移植、缺血再灌注損傷、炎症研究是氫氣生物學領域的主要研究內容。

放射損傷與自由基的關係是自由基生物學最早涉及的領域，抗自由基在放射損傷防護仍是目前的主流方法，國際上採用抗自由基對抗輻射損傷仍是臨床上的主要手段。

不過目前抗輻射損傷藥物由於存在非常明顯的毒性作用，限制了這些藥物的使用。氫氣由於沒有任何毒性作用，如果能用於抗輻射損傷，將是這個領域的重要發現，會對臨床上放射治療和放射診斷過程中引起的損傷的有效預防帶來重要貢獻。

目前，第二軍醫大學放射醫學研究已經先後發表了4篇使用氫氣溶液治療放射損傷的論文，證明氫氣能治療放射性淋巴細胞和腸細胞損傷，在國際上已經逐漸成為氫氣生物學方向的一個特色。

本研究主要是為了證明氫氣溶液對放射引起的心臟損傷保護效應的觀察，研究發現氫氣可顯著提高放射後動物生存率，並對放射引起的心臟損傷具有明顯的治療作用，同時能降低心臟氧化損傷，提高抗氧化酶水準。研究結果提示氫氣在放射性心臟損傷中具有潛在的臨床應用前景。

本研究研應用高壓灌注法定製含氫水，確保溶氫量大於 0.6 mmol/L，氫水製備後儲存於鋁箔袋保持密封狀態，並於四度低溫冷藏，確保含氫量，低溫靜置有助於氫氣於水中保存溶解。

氫水的製備方法可透過1.電解，2.水與礦石反應、3.高壓灌注，選擇任一方法均可備製水中含氫之氫水，任一方法皆可並無特殊性、惟成本與時間之差異。美國、日本、韓國、中國研究中使用之氫水，均因其實驗設計使用三種方式產生氫水，水中含氫氣即為氫水，檢測方法須應用溶氫檢測儀檢測之、只要水中含氫氣即為醫學研究所定義之氫水。

 Hydrogen-rich water production Hydrogen was dissolved in pure water 6 hours under high pressure (0.4 MPa) to a supersaturated level using hydrogenrich water-producing apparatus produced by our department. The saturated hydrogen water was stored under atmospheric pressure at 4°C in an aluminum bag with no dead volume. Hydrogen-rich water was freshly prepared every week, which ensured that a concentration of more than 0.6 mmol/L was maintained. Gas chromatography (Biogas Analyzer Systems-1000, Mitleben, Japan) was used to confirm the content of hydrogen in water by the method described by Ohsawa et al..11)

J Radiat Res (Tokyo). 2010;51(6):741-7.

The potential cardioprotective effects of hydrogen in irradiated mice.

Qian L, Cao F, Cui J, Wang Y, Huang Y, Chuai Y, Zaho L, Jiang H, Cai J.

Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University.

Abstract

Most ionizing radiation-induced damage is caused by hydroxyl radicals, and the selective reduction of hydroxyl by hydrogen in vitro has been demonstrated previously. Irradiation of the heart can cause chronic cardiac disease. This study was designed to test the hypothesis that hydrogen-rich water (pure water saturated with molecular hydrogen), which is easy to use, induces cardioprotection against ionizing irradiation injury in mice. In this paper, we demonstrate that hydrogen can protect myocardium degeneration from radiation-induced injury, decrease myocardium malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG) levels, and increase myocardium endogenous antioxidants in vivo. We suggest that hydrogen has a cardioprotective effect against radiation induced injury.

原文 pdf 

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